PRODUCT DESCRIPTION

Lactobacillus salivarius is a probiotic bacteria species that has been found to live in the gastrointestinal tract and exert a range of therapeutic properties including suppression of pathogenic bacteria Lactobacillus salivarius has been found to be of benefit in the alleviation of the symptom flatulence in individuals suffering from irritable bowel syndrome.

SPECIFICAITON

Strain name	Lactobacillus salivarius	Strain number	HH-LS17
Potency	200bn cfu/g	Gene Identification	Yes
Deposit	CCTCC	Certification	ISO,HACCP,HALAL,KOSHER
Packing	500g,1kg or 5kg per foil bag	Storage	-18℃ for long time, 2-8℃ for short time

TECHNICAL INDICATORS

Items		Specification
1) Live Lactobacillus salivarius potency		≥ 2.0×10¹¹ CFU/g
2) Water content		≤8.0%
4) Coliforms		≤10CFU/g
5) Moulds, Yeasts		≤10CFU/g
6) Pathogenic bacteria		Negative
7) Heavy metals	Lead (Pb)	≤1ppm
	Arsenic (As)	≤0.5ppm

RELEVANT RESEARCH

Strain Name

Identification

Deposit No.

Antibiotic Susceptibility

Acid and Bile Salt resistance

Stability

Quality System

L.salivarius HH-LS17

16S rRNA

Done

SC/ISO9001/ISO22000/FSSC22000/HACCP/

HALAL/KOSHER/FDA

ACID TOLERANCE

Acid resistance of Lactobacillus salivarius HH-LS17 powder was carried out at either pH=2.0 and pH=3.0 for up to 4 hours. The microbial enumeration of Lactobacillus salivarius HH-LS17 should be performed no more frequently than at the initial(as control), 1 hour, 2 hour, and 4 hour time points. The Fig 1 illustrates that Lactobacillus salivarius HH-LS17 can survive at low pH conditions, which means this strain has good acid resistance.

Fig 1. Acid resistance of L.salivarius HH-LS17

BILE SALT TOLERANCE

Bile salt resistance of Lactobacillus salivarius HH-LS17 powder was carried out at either 0.2% and 0.3% bile salt for up to 4 hours. The microbial enumeration of Lactobacillus salivarius HH-LS17 should be performed no more frequently than at the initial(as control), 1 hour, 2 hour, and 4 hour time points. The Fig 2 illustrates that Lactobacillus salivarius HH-LS17 can survive at high concentration of bile salt, which means this strain has good bile salt resistance.

Fig 2. Bile salt resistance of L.salivarius HH-LS17

STORAGE STABILITY

Lactobacillus salivarius HH-LS17 powder were sealed in aluminum foil bag, maintained at -18℃ for 24 months for long-term stability test. The microbial enumeration of Lactobacillus salivarius HH-LS17 should be performed no more frequently than at the initial, 1, 2, 3, 6, 9, 12, 15, 18, 21, 24 month time points. According to Fig 3, the potency of Lactobacillus salivarius HH-LS17 powder was 2.31×10¹¹CFU/g. After a storage period of 24 months, the potency of the powder was 1.17×10¹¹CFU/g. It means that this strain can remain stable for long time if stored at low temperature.