PRODUCT DESCRIPTION

Lactobacillus rhamnosus is a bacteria that exists naturally in the body, primarily in the intestines. It has been used as a probiotic, or "friendly bacteria" to prevent the growth of harmful bacteria in the stomach and intestines. It has been used in alternative medicine as a likely effective aid in treating or preventing diarrhea caused by rotavirus in babies and children.

SPECIFICAITON

Strain name	Lactobacillus rhamnosus	Strain number	PB-LR76
Potency	300bn cfu/g	Gene Identification	Yes
Deposit	CCTCC	Certification	ISO9001/ISO22000/FSSC22000/HACCP/HALAL/KOSHER/FDA
Packing	500g,1kg or 5kg per foil bag	Storage	-18℃ for long time, 2-8℃ for short time

TECHNICAL INDICATORS

Items		Specification
1) Live Lactobacillus rhamnosus potency		≥ 3.0×10¹¹ CFU/g
2) Water content		≤8.0%
4) Coliforms		≤10CFU/g
5) Moulds, Yeasts		≤10CFU/g
6) Pathogenic bacteria		Negative
7) Heavy metals	Lead (Pb)	≤1ppm
	Arsenic (As)	≤0.5ppm

RELEVANT RESEARCH

Strain Name

Identification

Deposit No.

Antibiotic Susceptibility

Acid and Bile Salt resistance

Stability

Quality System

L.rhamnosus PB-LR76

16S rRNA

Done

SC/ISO9001/ISO22000/FSSC22000/HACCP/

HALAL/KOSHER/FDA

ACID TOLERANCE

Acid resistance of Lactobacillus rhamnosus PB-LR76 powder was carried out at either pH=2.0 and pH=3.0 for up to 4 hours. The microbial enumeration of Lactobacillus rhamnosus PB-LR76 should be performed no more frequently than at the initial(as control), 1 hour, 2 hour, and 4 hour time points. The Fig 1 illustrates that Lactobacillus rhamnosus PB-LR76 can survive at low pH conditions, which means this strain has good acid resistance.

Fig 1. Acid resistance of L.rhamnosus PB-LR76

BILE SALT TOLERANCE

Bile salt resistance of Lactobacillus rhamnosus PB-LR76 powder was carried out at either 0.2% and 0.3% bile salt for up to 4 hours. The microbial enumeration of Lactobacillus rhamnosus PB-LR76 should be performed no more frequently than at the initial(as control), 1 hour, 2 hour, and 4 hour time points. The Fig 2 illustrates that Lactobacillus rhamnosus PB-LR76 can survive at high concentration of bile salt, which means this strain has good bile salt resistance.

Fig 2. Bile salt resistance of L.rhamnosus PB-LR76

STORAGE STABILITY

Lactobacillus rhamnosus PB-LR76 powder were sealed in aluminum foil bag, maintained at -18℃ for 24 months for long-term stability test. The microbial enumeration of Lactobacillus rhamnosus PB-LR76 should be performed no more frequently than at the initial, 1, 2, 3, 6, 9, 12, 15, 18, 21, 24 month time points. According to Fig 3, the potency of Lactobacillus rhamnosus PB-LR76 powder was 3.61×10¹¹CFU/g. After a storage period of 24 months, the potency of the powder was 2.18×10¹¹CFU/g. It means that this strain can remain stable for long time if stored at low temperature.