Product Description

Lactobacillus reuteri was first isolated in 1962 and is a well-studied probiotic bacterium that can colonize a large number of mammals. It is found in various parts of the human body sites, including the gastrointestinal tract, urinary tract, skin, and breast milk.

Specificaiton

Strain name	Lactobacillus reuteri	Strain number	PB-LR09
Potency	200bn cfu/g	Gene Identification	Yes
Deposit	CCTCC	Certification	ISO9001/ISO22000/FSSC22000/HACCP/HALAL/KOSHER/FDA
Packing	500g,1kg or 5kg per foil bag	Storage	-18℃ for long time, 2-8℃ for short time

TECHNICAL INDICATORS

Items		Specification
1) Live Lactobacillus reuteri potency		≥ 2.0×10¹¹ CFU/g
2) Water content		≤8.0%
4) Coliforms		≤10CFU/g
5) Moulds, Yeasts		≤10CFU/g
6) Pathogenic bacteria		Negative
7) Heavy metals	Lead (Pb)	≤1ppm
	Arsenic (As)	≤0.5ppm

RELEVANT RESEARCH

Strain Name

Identification

Deposit No.

Antibiotic Susceptibility

Acid and Bile Salt resistance

Stability

Quality System

L.reuteri PB-LR09

16S rRNA

Done

SC/ISO9001/ISO22000/FSSC22000/HACCP/

HALAL/KOSHER/FDA

ACID TOLERANCE

Acid resistance of Lactobacillus reuteri PB-LR09 powder was carried out at either pH=2.0 and pH=3.0 for up to 4 hours. The microbial enumeration of Lactobacillus reuteri PB-LR09 should be performed no more frequently than at the initial(as control), 1 hour, 2 hour, and 4 hour time points. The Fig 1 illustrates that Lactobacillus reuteri PB-LR09 can survive at low pH conditions, which means this strain has good acid resistance.

Fig 1. Acid resistance of L.reuteri PB-LR09

BILE SALT TOLERANCE

Bile salt resistance of Lactobacillus reuteri PB-LR09 powder was carried out at either 0.2% and 0.3% bile salt for up to 4 hours. The microbial enumeration of Lactobacillus reuteri PB-LR09 should be performed no more frequently than at the initial(as control), 1 hour, 2 hour, and 4 hour time points. The Fig 2 illustrates that Lactobacillus reuteri PB-LR09 can survive at high concentration of bile salt, which means this strain has good bile salt resistance.

Fig 2. Bile salt resistance of L.reuteri PB-LR09

STORAGE STABILITY

Lactobacillus reuteri PB-LR09 powder were sealed in aluminum foil bag, maintained at -18℃ for 24 months for long-term stability test. The microbial enumeration of Lactobacillus reuteri PB-LR09 should be performed no more frequently than at the initial, 1, 2, 3, 6, 9, 12, 15, 18, 21, 24 month time points. According to Fig 3, the potency of Lactobacillus reuteri PB-LR09 powder was 2.77×10¹¹CFU/g. After a storage period of 24 months, the potency of the powder was 1.75×10¹¹CFU/g. It means that this strain can remain stable for long time if stored at low temperature.