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Product Description

Lactobacillus bulgaricus HH-LB57 is a beneficial bacteria found in the digestive tract. It is the main bacterium used for the production of yogurt. It also plays a crucial role in the ripening of some cheeses, as well as in other processes involving naturally fermented products. It is defined as homofermentive lactic acid bacteria due to lactic acid being the single end product of its carbohydrate digestion. It is also considered a probiotic.

SPECIFICAITON

Strain name	Lactobacillus bulgaricus	Strain number	HH-LB57
Potency	50bn cfu/g	Gene Identification	Yes
Deposit	CCTCC	Certification	ISO9001/ISO22000/FSSC22000/HACCP/HALAL/KOSHER/FDA
Packing	500g,1kg or 5kg per foil bag	Storage	-18℃ for long time, 2-8℃ for short time

TECHNICAL INDICATORS

Items		Specification
1) Live Lactobacillus bulgaricus potency		≥ 5.0×10¹⁰CFU/g
2) Water content		≤8.0%
4) Coliforms		≤10CFU/g
5) Moulds, Yeasts		≤10CFU/g
6) Pathogenic bacteria		Negative
7) Heavy metals	Lead (Pb)	≤1ppm
	Arsenic (As)	≤0.5ppm

RELEVANT RESEARCH

Strain Name

Identification

Deposit No.

Antibiotic Susceptibility

Acid and Bile Salt resistance

Stability

Quality System

L.bulgaricus HH-LB57

16S rRNA

Done

SC/ISO9001/ISO22000/FSSC22000/HACCP/

HALAL/KOSHER/FDA

ACID TOLERANCE

Acid resistance of Lactobacillus bulgaricus HH-LB57 powder was carried out at either pH=2.0 and pH=3.0 for up to 4 hours. The microbial enumeration of Lactobacillus bulgaricus HH-LB57 should be performed no more frequently than at the initial(as control), 1 hour, 2 hour, and 4 hour time points. The Fig 1 illustrates that Lactobacillus bulgaricus HH-LB57 can survive at low pH conditions, which means this strain has good acid resistance.

Fig 1. Acid resistance of L.bulgaricus HH-LB57

BILE SALT TOLERANCE

Bile salt resistance of Lactobacillus bulgaricus HH-LB57 powder was carried out at either 0.2% and 0.3% bile salt for up to 4 hours. The microbial enumeration of Lactobacillus bulgaricus HH-LB57 should be performed no more frequently than at the initial(as control), 1 hour, 2 hour, and 4 hour time points. The Fig 2 illustrates that Lactobacillus bulgaricus HH-LB57 can survive at high concentration of bile salt, which means this strain has good bile salt resistance.

Fig 2. Bile salt resistance of L.bulgaricus HH-LB57

STORAGE STABILITY

Lactobacillus bulgaricus HH-LB57 powder were sealed in aluminum foil bag, maintained at -18℃ for 24 months for long-term stability test. The microbial enumeration of Lactobacillus bulgaricus HH-LB57 should be performed no more frequently than at the initial, 1, 2, 3, 6, 9, 12, 15, 18, 21, 24 month time points. According to Fig 3, the potency of Lactobacillus bulgaricus HH-LB57 powder was 5.00×10¹⁰CFU/g. After a storage period of 24 months, the potency of the powder was 3.30×10¹⁰CFU/g. It means that this strain can remain stable for long time if stored at low temperature.

Fig 3. Storage stability of L.bulgaricus HH-LB57

HEALTH BENEFITS

Growth Inhibition of Pathogenic Bacteria

The aim of this study was evaluation of antagonistic effect of L.bulgaricus HH-LB57 and its fermentation supernatant against four pathogens. Antimicrobial effects of isolates were evaluated by disc diffusion test on plates with different pathogens. Growth inhibition zones of pathogens and L. bulgaricus HH-LB57 inhibitory ability were assessed after incubation at 37℃ for 48h. The results showed(Table 1) that L.bulgaricus HH-LB57 had relatively strong activity against tested pathogens (with inhibition zone diameter> 15mm).

Pathogens	L.bulgaricus HH-LB57 cells (Inhibition zone’s diameter/mm)	Fermentation supernatant (Inhibition zone’s diameter/mm)
S.epidermidis	22.5±4.8	22.6±4.0
E.coli	16.5±3.6	16.1±3.4
E. aerogenes	23.6±4.1	20.5±5.9
S.aureus	15.2±2.1	11.4±2.2