PRODUCT DESCRIPTION

Lactobacillus casei is one of the many species of bacteria belonging in the genus Lactobacillus. It is a mesophilic bacteria that is gram positive, rod shaped, nonsporing, nonmotile, anaerobic, and contains no cytochromes. L. casei can be found in various environments such as raw and fermented dairy products, intestinal tracts and reproductive systems of humans and animals, and fresh and fermented plant products. The optimum pH for L. casei is 5.5. The lactic acid produced by L. casei through fermentation is very important since it can be used to make cheeses and yogurts, reduce cholesterol levels, enhance immune response, control diarrhea, alleviate lactose intolerance, inhibit intestinal pathogens, and serve as probiotics. Probiotics are viable microorganisms that promote or support a beneficial balance of microbes to live in the gastrointestinal tract .

SPECIFICAITON

Strain name	Lactobacillus casei	Strain number	PB-LC39
Potency	300bn cfu/g	Gene Identification	Yes
Deposit	CCTCC	Certification	ISO9001/ISO22000/FSSC22000/HACCP/HALAL/KOSHER/FDA
Packing	500g,1kg or 5kg per foil bag	Storage	-18℃ for long time, 2-8℃ for short time

TECHNICAL INDICATORS

Items		Specification
1) Live Lactobacillus casei potency		≥ 3.0×10¹¹ CFU/g
2) Water content		≤8.0%
4) Coliforms		≤10CFU/g
5) Moulds, Yeasts		≤10CFU/g
6) Pathogenic bacteria		Negative
7) Heavy metals	Lead (Pb)	≤1ppm
	Arsenic (As)	≤0.5ppm

RELEVANT RESEARCH

Strain Name

Identification

Deposit No.

Antibiotic Susceptibility

Acid and Bile Salt resistance

Stability

Quality System

L.casei PB-LC39

16S rRNA

CCTCC NO:M2018886

Done

SC/ISO9001/ISO22000/FSSC22000/HACCP/

HALAL/KOSHER/FDA

ACID TOLERANCE

Acid resistance of Lactobacillus casei PB-LC39 powder was carried out at either pH=2.0 and pH=3.0 for up to 4 hours. The microbial enumeration of Lactobacillus casei PB-LC39 should be performed no more frequently than at the initial(as control), 1 hour, 2 hour, and 4 hour time points. The Fig 1 illustrates that Lactobacillus casei PB-LC39 can survive at low pH conditions, which means this strain has good acid resistance.

Fig 1. Acid resistance of L.casei PB-LC39

BILE SALT TOLERANCE

Bile salt resistance of Lactobacillus casei PB-LC39 powder was carried out at either 0.2% and 0.3% bile salt for up to 4 hours. The microbial enumeration of Lactobacillus casei PB-LC39 should be performed no more frequently than at the initial(as control), 1 hour, 2 hour, and 4 hour time points. The Fig 2 illustrates that Lactobacillus casei PB-LC39 can survive at high concentration of bile salt, which means this strain has good bile salt resistance.

Fig 2. Bile salt resistance of L.casei PB-LC39

STORAGE STABILITY

Lactobacillus casei PB-LC39 powder were sealed in aluminum foil bag, maintained at -18℃ for 24 months for long-term stability test. The microbial enumeration of Lactobacillus casei PB-LC39 should be performed no more frequently than at the initial, 1, 2, 3, 6, 9, 12, 15, 18, 21, 24 month time points. According to Fig 3, the potency of Lactobacillus casei PB-LC39 powder was 2.87×10¹¹CFU/g. After a storage period of 24 months, the potency of the powder was 2.18×10¹¹CFU/g. It means that this strain can remain stable for long time if stored at low temperature.

Fig 3. Storage stability of L.casei PB-LC39

HEALTH BENEFITS

1. Immunomodulation

Natural killer (NK) cells are lymphocytes of the innate immune system. These cells play important roles in immunity against viruses and in the immune surveillance of tumors.After 30-days intragastric administration of L. casei PB-LC39 in mice, the results(Fig 4.) showed that L.casei PB-LC39 significantly increase cytotoxicity of NK cells in a dose-dependent manner.

Fig 4. L. casei PB-LC39’s effects on cytotoxicity of NK cells

2. Reduce oxidative stress in liver

Oxidative stress is a phenomenon caused by an imbalance between production and accumulation of oxygen reactive species(ROS) in cells and tissues and the ability of a biological system to detoxify these reactive products. Oxidative stress indicators including superoxide dismutase(SOD) and malondialdehyde(MDA) were tested in the animal experiment. The results (Table 2) showed that alcohol can induce oxidative stress in liver and after L. casei PB-LC39 treatment, SOD and MDA restored to nomal level, which means L. casei PB-LC39(Table 1.) can reduce oxidative stress in liver.

Oxidative stress indicators	SOD, U/mgprot	MDA,U/mgprot
Control group	48.82±2.96	0.75±0.05
Alcohol treatment（mM）	1.0	32.74±1.31	1.33±0.10
L. casei PB-LC39 treatment（cfu/mL）	1.0×10⁵	31.69±0.95	1.39±0.19
	1.0×10⁶	36.78±0.77	1.06±0.12
	1.0×10⁷	42.14±1.49	0.99±0.02
	1.5×10⁷	42.34±2.12	0.98±0.06

Table 1. Effects of L. casei PB-LC39 treatment in liver oxidative stress

3. Reduce cholesterol level

Total cholesterol is the total amount of cholesterol in blood. In experiment, we found that L. casei PB-LC39 (Table 2.) treatment can reduce total cholesterol level comparing to initial level and placebo treatment.

	Initial		6weeks		12weeks
	Placebo	L.casei	Placebo	L.casei	Placebo	L.casei
>2500 mg/l TC（mg/l）	2703	2769	2550	2555	2574	2286

<2500 mg/l TC（mg/l）	2295	2217	2189	2131	2219	2008