PRODUCT DESCRIPTION

Lactobacillus crispatus HH-LC23 is a common, rod-shaped species of genus Lactobacillus and is a hydrogen peroxide producing beneficial microbiota species located in both the vagina, through vaginal discharge, and the vertebrate gastrointestinal tract. The strain can be used by premenopausal and postmenopausal women that experience recurrent urinary tract infections. It is being evaluated specifically for the prevention and treatment of bacterial vaginosis, which is characterized by the absence of lactobacillus flora necessary to protect the host.

SPECIFICAITON

Strain name	Lactobacillus crispatus	Strain number	HH-LC23
Potency	100bn cfu/g	Gene Identification	Yes
Deposit	CCTCC	Certification	ISO9001/ISO22000/FSSC22000/HACCP/HALAL/KOSHER/FDA
Packing	500g,1kg or 5kg per foil bag	Storage	-18℃ for long time, 2-8℃ for short time

TECHNICAL INDICATORS

Items		Specification
1) Live Lactobacillus crispatus potency		≥ 1.0×10¹¹ CFU/g
2) Water content		≤8.0%
4) Coliforms		≤10CFU/g
5) Moulds, Yeasts		≤10CFU/g
6) Pathogenic bacteria		Negative
7) Heavy metals	Lead (Pb)	≤1ppm
	Arsenic (As)	≤0.5ppm

RELEVANT RESEARCH

Strain Name

Identification

Deposit No.

Antibiotic Susceptibility

Acid and Bile Salt resistance

Stability

Quality System

L.crispatus HH-LC23

16S rRNA

Done

SC/ISO9001/ISO22000/FSSC22000/HACCP/

HALAL/KOSHER/FDA

ACID TOLERANCE

Acid resistance of Lactobacillus crispatus HH-LC23 powder was carried out at either pH=2.0 and pH=3.0 for up to 4 hours. The microbial enumeration of Lactobacillus crispatus HH-LC23 should be performed no more frequently than at the initial(as control), 1 hour, 2 hour, and 4 hour time points. The Fig 1 illustrates that Lactobacillus crispatus HH-LC23 can survive at low pH conditions, which means this strain has good acid resistance.

Fig 1. Acid resistance of L.crispatus HH-LC23

BILE SALT TOLERANCE

Bile salt resistance of Lactobacillus crispatus HH-LC23 powder was carried out at either 0.2% and 0.3% bile salt for up to 4 hours. The microbial enumeration of Lactobacillus crispatus HH-LC23 should be performed no more frequently than at the initial(as control), 1 hour, 2 hour, and 4 hour time points. The Fig 2 illustrates that Lactobacillus crispatus HH-LC23 can survive at high concentration of bile salt, which means this strain has good bile salt resistance.

Fig 2. Bile salt resistance of L.crispatus HH-LC23

STORAGE STABILITY

Lactobacillus crispatus HH-LC23 powder were sealed in aluminum foil bag, maintained at -18℃ for 24 months for long-term stability test. The microbial enumeration of Lactobacillus crispatus HH-LC23 should be performed no more frequently than at the initial, 1, 2, 3, 6, 9, 12, 15, 18, 21, 24 month time points. According to Fig 3, the potency of Lactobacillus crispatus HH-LC23 powder was 1.50×10¹¹CFU/g. After a storage period of 24 months, the potency of the powder was 1.01×10¹¹CFU/g. It means that this strain can remain stable for long time if stored at low temperature.