PRODUCT DESCRIPTION

Lactobacillus acidophilus is a species of Gram-positive bacteria in the genus Lactobacillus. L. acidophilus is a homofermentative, microaerophilic species, fermenting sugars into lactic acid, and grows readily at rather low pH values (below pH 5.0) and has an optimum growth temperature of around 37 ℃. L. acidophilus is found in the human and animal gastrointestinal tract and mouth. It is commercially used in many dairy products, sometimes together with Streptococcus thermophilus and Lactobacillus delbrueckii subsp. bulgaricus in the production of acidophilus-type yogurt, or acidophiline.

SPECIFICAITON

Strain name	Lactobacillus acidophilus	Strain number	HH-LA26
Potency	200bn cfu/g	Gene Identification	Yes
Deposit	CCTCC	Certification	ISO9001/ISO22000/FSSC22000/HACCP/HALAL/KOSHER/FDA
Packing	500g,1kg or 5kg per foil bag	Storage	-18℃ for long time, 2-8℃ for short time

TECHNICAL INDICATORS

Items		Specification
1) Live Lactobacillus acidophilus potency		≥ 2.0×10¹¹ CFU/g
2) Water content		≤8.0%
4) Coliforms		≤10CFU/g
5) Moulds, Yeasts		≤10CFU/g
6) Pathogenic bacteria		Negative
7) Heavy metals	Lead (Pb)	≤1ppm
	Arsenic (As)	≤0.5ppm

RELEVANT RESEARCH

Strain Name

Identification

Deposit No.

Antibiotic Susceptibility

Acid and Bile Salt resistance

Stability

Quality System

L.acidophilus HH-LA26

16S rRNA

Done

SC/ISO9001/ISO22000/FSSC22000/HACCP/

HALAL/KOSHER/FDA

ACID TOLERANCE

Acid resistance of Lactobacillus acidophilus HH-LA26 powder was carried out at either pH=2.0 and pH=3.0 for up to 4 hours. The microbial enumeration of Lactobacillus acidophilus HH-LA26 should be performed no more frequently than at the initial(as control), 1 hour, 2 hour, and 4 hour time points. The Fig 1 illustrates that Lactobacillus acidophilus HH-LA26 can survive at low pH conditions, which means this strain has good acid resistance.

Fig 1. Acid resistance of L.acidophilus HH-LA26

BILE SALT TOLERANCE

Bile salt resistance of Lactobacillus acidophilus HH-LA26 powder was carried out at either 0.2% and 0.3% bile salt for up to 4 hours. The microbial enumeration of Lactobacillus acidophilus HH-LA26 should be performed no more frequently than at the initial(as control), 1 hour, 2 hour, and 4 hour time points. The Fig 2 illustrates that Lactobacillus acidophilus HH-LA26 can survive at high concentration of bile salt, which means this strain has good bile salt resistance.

Fig 2. Bile salt resistance of L.acidophilus HH-LA26

STORAGE STABILITY

Lactobacillus acidophilus HH-LA26 powder were sealed in aluminum foil bag, maintained at -18℃ for 24 months for long-term stability test. The microbial enumeration of Lactobacillus acidophilus HH-LA26 should be performed no more frequently than at the initial, 1, 2, 3, 6, 9, 12, 15, 18, 21, 24 month time points. According to Fig 3, the potency of Lactobacillus acidophilus HH-LA26 powder was 3.30×10¹¹CFU/g. After a storage period of 24 months, the potency of the powder was 2.14×10¹¹CFU/g. It means that this strain can remain stable for long time if stored at low temperature.

Fig 3. Storage stability of L.acidophilus HH-LA26

HEALTH BENEFITS

1. Growth Inhibition of Pathogenic Bacteria

The aim of this study was evaluation of antagonistic effect of L.acidophilus HH-LA26 against four pathogens. Antimicrobial effects of isolates were evaluated by disc diffusion test on plates with different pathogens. Growth inhibition zones of pathogens and L. acidophilus HH-LA26 inhibitory ability were assessed after incubation of all agar media at 37℃ for 24h. The results showed(Table 1) that L.acidophilus HH-LA26 had relatively strong activity against tested pathogens (with inhibition zone diameter> 15mm).

Pathogens	Culture medium	Inhibition zone’s diameter/mm
E.coli	LB agar	34.88±0.17
Salmonella ssp	LB agar	36.52±0.22
Staphylococcus aureus	Nutrient agar	40.33±0.27
Listeria monocytogenes	BHI agar	29.74±0.15

Table 1. L. acidophilus HH-LA26 inhibitory ability

2. Modulate Immune System

After 30-days intragastric administration of L. acidophilus HH-LA26 in mice, the results(Fig 4.) showed that L.acidophilus HH-LA26 significantly increase proliferation of splenic lymphocyte in a dose-dependent manner.

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