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PRODUCT DESCRIPTION

Lactobacillus fermentum is a Gram-positive species of bacterium in the genus Lactobacillus. It is located naturally in the mouth, gastrointestinal tract, and vaginal tract in humans. It is more survivable in acid and bile than other probiotic strains. L. fermentum offers a number of potential benefits, including reducing cholesterol and boosting immunity. It is also one of the few strains particularly beneficial for dealing with issues in women like yeast infections and UTIs

SPECIFICAITON

Strain name	Lactobacillus fermentum	Strain number	HH-LF39
Potency	200bn cfu/g	Gene Identification	Yes
Deposit	CCTCC	Certification	ISO9001/ISO22000/FSSC22000/HACCP/HALAL/KOSHER/FDA
Packing	500g,1kg or 5kg per foil bag	Storage	-18℃ for long time, 2-8℃ for short time

TECHNICAL INDICATORS

Items		Specification
1) Live Lactobacillus fermentum potency		≥ 2.0×10¹¹ CFU/g
2) Water content		≤8.0%
4) Coliforms		≤10CFU/g
5) Moulds, Yeasts		≤10CFU/g
6) Pathogenic bacteria		Negative
7) Heavy metals	Lead (Pb)	≤1ppm
	Arsenic (As)	≤0.5ppm

RELEVANT RESEARCH

Strain Name

Identification

Deposit No.

Antibiotic Susceptibility

Acid and Bile Salt resistance

Stability

Quality System

L.fermentum HH-LF39

16S rRNA

Done

SC/ISO9001/ISO22000/FSSC22000/HACCP/

HALAL/KOSHER/FDA

ACID TOLERANCE

Acid resistance of Lactobacillus fermentum HH-LF39 powder was carried out at either pH=2.0 and pH=3.0 for up to 4 hours. The microbial enumeration of Lactobacillus fermentum HH-LF39 should be performed no more frequently than at the initial(as control), 1 hour, 2 hour, and 4 hour time points. The Fig 1 illustrates that Lactobacillus fermentum HH-LF39 can survive at low pH conditions, which means this strain has good acid resistance.

Fig 1. Acid resistance of L.fermentum HH-LF39

BILE SALT TOLERANCE

Bile salt resistance of Lactobacillus fermentum HH-LF39 powder was carried out at either 0.2% and 0.3% bile salt for up to 4 hours. The microbial enumeration of Lactobacillus fermentum HH-LF39 should be performed no more frequently than at the initial(as control), 1 hour, 2 hour, and 4 hour time points. The Fig 2 illustrates that Lactobacillus fermentum HH-LF39 can survive at high concentration of bile salt, which means this strain has good bile salt resistance.

Fig 2. Bile salt resistance of L.fermentum HH-LF39

STORAGE STABILITY

Lactobacillus fermentum HH-LF39 powder were sealed in aluminum foil bag, maintained at -18℃ for 24 months for long-term stability test. The microbial enumeration of Lactobacillus fermentum HH-LF39 should be performed no more frequently than at the initial, 1, 2, 3, 6, 9, 12, 15, 18, 21, 24 month time points. According to Fig 3, the potency of Lactobacillus fermentum HH-LF39 powder was 2.33×10¹¹CFU/g. After a storage period of 24 months, the potency of the powder was 1.32×10¹¹CFU/g. It means that this strain can remain stable for long time if stored at low temperature.

Fig 3. Storage stability of L.fermentum HH-LF39

APPLICATIONS

Dietary supplement, food and drink, dairy products fermentation and other fermented food. It can also be used for food preservation and biomedical applications.