Sleep Aid Probiotics Capsule
Specifications: 0.5g×60 capsules/box
Formula (content per capsule):
Lactobacillus plantarum N-1: 50 Billion CFU
Rhamnosus PB-LR76: 30 Billion CFU
Lactobacillus paracasei HH-LP58: 40 Billion CFU
Chamomile: 40 mg, Lemon Balm 40 mg, Passionflower: 40 mg, Ziziphus Seed: 40 mg, Galacto-oligosaccharide: 40 mg
This probiotic formula is customized to support sleep. This probiotic formula contains patented and scientifically proven probiotic strains Lactobacillus plantarum N-1 and Rhamnosus PB-LR76, which have been scientifically proven to:
√Relieve stress
√ Eliminate inflammation
Recommended usage and dosage: 1 capsule per day, taken orally with warm water 30 minutes before going to bed.
Note: Improving sleep is a long-term process and it is recommended to take it every day. After taking it, please lie down and close your eyes. Do not play with your mobile phone, watch TV series, play games, or other behaviors that will excite your brain nerves, so as not to affect the effect. In addition, try to fix your daily work and rest time, which can easily form a perfect biological clock and lengthen the time of deep sleep. The effect of improving sleep is better.
Storage method: Store in a cool place, preferably at 4°C.
Shelf life: 24 months
The mechanism by which intestinal flora affects sleep
According to research on the gut-brain axis mechanism in the past decade or so, scientists have found that intestinal flora can mainly affect sleep through the following four pathways:
1. Immune system pathway
Both the brain and gut microbiomes influence the activity of immune cells and, in turn, influence each other. Intestinal bacteria are engulfed and digested by phagocytes (such as macrophages or neutrophils); the digestion products (such as MPs (mononuclear phagocyte system), LPS (bacterial lipopolysaccharide)) are released into the surrounding intercellular space liquid. MPs and LPS in turn activate phagocytes, which then release cytokines.
2. Neuroendocrine pathway
There are more than 20 types of enteroendocrine cells in the intestine, making up the largest endocrine organ. The gut microbiota is directly involved in the production of multiple neurotransmitters, cytokines, and metabolites, such as 5-HT (serotonin), dopamine, gamma-aminobutyric acid (GABA), SCFA (short-chain fatty acids), and melatonin Hormones, etc. Certain Lactobacilli and Bifidobacteria can produce GABA. Abnormal expression of GABA mRNA is frequently observed in patients with insomnia. E. coli produces norepinephrine, serotonin, and dopamine; Streptococcus and Enterococcus produce serotonin; and Bacillus produces norepinephrine and dopamine. Beneficial bacteria are involved in the secretion of hormones, which trigger drowsiness. Tryptophan in the amino acid is an essential raw material for the synthesis of melatonin because our body cannot produce it on its own and must obtain it from food. The protein in food must also be broken down into tryptophan by bacteria in the intestines. Tryptophan forms 5-hydroxytryptamine, which is then transported to the brain and converted into the neurotransmitter serotonin. Serotonin will form the hormone melatonin. Therefore, if the bacteria in the intestines cannot function normally, they will affect the secretion of melatonin, leading to a lack of sleep.
3. Hypothalamic-pituitary-adrenal (HPA) axis pathway
The HPA axis is involved in homeostasis and responses to new stimuli. The HPA axis is an adaptive system designed to maintain homeostasis in a changing environment. A growing body of research demonstrates a correlation between sleep and HPA axis activity. Hyperactivity of the HPA axis can negatively impact sleep, leading to sleep fragmentation, reduced deep slow-wave sleep, and shorter sleep duration. In turn, sleep disorders including insomnia and obstructive sleep apnea can further exacerbate HPA axis dysfunction.
4. Vagus nerve pathway
Sensory neurons of the myenteric plexus engage the gut microbiota by regulating intestinal motility and intestinal hormone secretion. Sensory neurons of the myenteric plexus engage the gut microbiota by regulating intestinal motility and intestinal hormone secretion. The enteric nervous system also forms synaptic connections with the vagus nerve, which connects the gut to the brain. Cytokines signal to the brain via vagal afferents, and action potentials from the vagus nerve further induce glial cells and neurons to produce cytokines in the brain. The level of cytokine concentration is related to sleep. Low concentrations of brain cytokines can promote sleep, while high concentrations of brain cytokines are detrimental to sleep.
Mechanism of action of probiotics
1. Participate in immune regulation
Probiotics can produce various cytokines and chemokines in the body and change peripheral inflammatory markers and the immune environment. The intestinal microbiota interacts with immune cells in the brain, thereby affecting the levels of cytokines, cell motility response factors, and prostaglandin E2, affecting the immune system of the host brain, regulating brain activity, and achieving the effect of promoting sleep.
2. Participate in regulating endocrine
Probiotics can modulate central neurotransmitters by changing the precursor levels of central neurotransmitters. Probiotics can also directly synthesize and release the neurotransmitter γ-aminobutyric acid (GABA). (GABA) is an important inhibitory neurotransmitter in the human central nervous system. It can inhibit the excitement of the sympathetic nervous system and act as a hypnotic agent. , sedation, and other effects.
3. Regulate the HPA axis and reduce nighttime excitement
Probiotics and prebiotics can act on intestinal microorganisms to regulate neural signals in the hypothalamic-pituitary-adrenal (HPA) axis through cortisol.
Lactobacillus plantarum N-1[2]
Source of bacteria: Yak cheese from the Qinghai-Tibet Plateau
Collection number: CGMCC No.15463
Acid and bile salt tolerance[3]
Acid and bile tolerance are the most important properties of probiotics as they determine their ability to survive in the small intestine and thus exert their functional role as a probiotic. N-1 showed very good survival at low pH and high bile salt concentration.
Adhesion[4]
The ability to adhere to the host is an important criterion for screening potential probiotics. It is thought to be associated with colonization, pathogen suppression, immune interactions, and enhancement of barrier function. The adhesion test results showed that the adhesion number of N-1 to Caco-2 cells was 6.04×106 CFU/mL, and the adhesion rate was 4.03%.
Note: The Caco-2 cell model is a human cloned colon adenocarcinoma cell. Its structure and function are similar to differentiated small intestinal epithelial cells. It has structures such as microvilli and contains enzyme systems related to the brush border epithelium of the small intestine. It can be used to Perform experiments simulating intestinal transport in vivo.
Sleep aid effect experiment
Fifty mice, weighing 18-22g, were randomly divided into 5 groups, namely Example 1-3 groups, a blank control group, and a positive control group. There were 10 mice in each group, half male and half female, and each group was administered intragastrically. The intragastric dosages were as follows: Examples 1-3 were administered 2g/kg of the sleep-promoting probiotic composition; the positive control group was administered 2g/kg of jujube kernel extract (purchased from Guangzhou Hecheng Sanxian Biotechnology Co., Ltd.); and the blank control was administered. The same amount of normal saline was given to the group. Administer once a day for 1 week. One week later, the monitoring camera was used to observe and record: the sleep rate of each group of mice after 30 minutes of intragastric administration; the sleep time of each group of mice within 12 hours after the end of intragastric administration, and the levels of 5-HT and 5-HT in the brains of each group of mice were measured. GABA content.
The mice in the probiotic group fed daily had a significant sleep-promoting effect, and the levels of GABA and 5-HT (5-hydroxytryptamine) in the brain were significantly increased.
Post-sleep state assessment
Thirty depression model rats were randomly divided into two groups. The control group was fed ordinary feed, and the experimental group was fed feed containing probiotics. The other feeding conditions were the same. The post-sleep mental scores of the two groups of mice were measured for 7 weeks to simulate human post-sleep recovery under high chronic stress. The weekly sleep duration and sleep time in the next 6 weeks were compared and evaluated.
The results showed that the health status of mice in the probiotic group was significantly improved after sleeping, and their state recovery after sleeping was also significantly improved. Moreover, the sleep latency of mice fed probiotics was shortened by 18.8%, and the sleep time increased by 7.2%.
sleep quality assessment
N-1 can significantly improve anxiety, stress, and depression-like behaviors in autistic mice
Raw materials from the same origin as medicine and food:
Chamomile: German Chamomile (German Chamomile) is native to Europe and belongs to the genus Matricaria of the Asteraceae family. It is an overwintering annual herb. The inflorescence has a light apple fragrance and has the effects of improving sleep, calming mood, nourishing the liver, and lowering blood pressure.
Lemon balm: In pharmacopeia and traditional medicine, lemon balm is used to treat gastrointestinal diseases and as a sedative to treat sleep disorders; in folk medicine, it is used to treat coughs, dizziness, dysmenorrhea, skin infections, and as a sedative, Antispasmodic, antiseptic.
Passionflower: Passionflower extract enhances affinity for GABA receptors and inhibits GABA reuptake in rat cortical synaptosomes; induces direct GABA currents in CA1 hippocampal pyramidal neurons, similar to benzodiazepines effects of drugs.
Ziziphus seeds: Ziziphus seeds contain various pharmacological ingredients such as alkaloids, saponins, and flavonoids. It has the effects of sedation, hypnosis, analgesia, anti-convulsant, lowering blood lipids, anti-platelet aggregation, enhancing immune function, reducing blood pressure, anti-hypoxia, anti-myocardial ischemia, anti-arrhythmia, anti-aging, and anti-radiation.
Articles & Patents
[1] Qiu Z, et al. (2023) Lactiplantibacillus plantarum N-1 improves autism-like behavior and gut microbiota in mice. Frontiers in Microbiology. DOI 10.3389/fmicb.2023.1134517
[2] Sen, Paromita, et al. "Microbiota and sleep: awakening the gut feeling." Trends in molecular medicine vol. 27,10 (2021): 935-945. doi:10.1016/j.molmed.2021.07.004
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