Anti Inflammation Probiotics

Probioway Co., Ltd. is one of the leading manufacturers and suppliers of anti inflammation probiotics in China, also supports custom service. Please feel free to wholesale bulk high quality anti inflammation probiotics from our factory. Good service and reasonable price are available.

1. Product introduction

• Product name: Anti inflammation probiotics
• Product specifications: 30 capsules/box
• Product weight: 0.5 g/tablet
• Instructions for taking: 0.5 g/tablet, 2 pills each time, once/d, continuous medication.
• Preservation method: Store in a cool and dry place, preferably refrigerated at 4°C.
• Viable bacterial count: When leaving the factory, the viable bacterial count is ≥ 400 billion CFU/box, containing Lactobacillus johnsonii LBJ 456 (200 billion CFU/box).
• Other ingredients: mulberries, jack beans, almonds, inulin, and fructooligosaccharides.

2. Probiotics and Arthritis

Studies have shown that intestinal microbial disorders can increase the permeability of the intestinal wall mucosa, causing intestinal microorganisms and metabolites to penetrate the outside of the intestine, and induce local colonization in the joint cavity with blood circulation under the induction of chemokines and adhesion molecules, inducing synovial inflammation. reaction. The abundance and diversity of the gut microbiota decrease as arthritis progresses, and intestinal disturbances can further exacerbate the inflammatory response. Supplementing probiotics can not only improve patients' joint disease activity scores and reduce C-reactive protein levels but also enhance the activity of regulatory T cells (Treg) in arthritic mice and reduce the release of inflammatory factors. Therefore, regulating intestinal flora has become a new direction in the treatment of arthritis.

Table 1 Common treatments for arthritis

3. Mechanism of probiotics regulating arthritis

Probiotics can reduce intestinal permeability, influence the immune system, enhance the distal secretory immunoglobulin A (IgA) immune response to pathogens, limit the proliferation of bacterial strains, and reduce activated immune components such as tumor necrosis factor α (TNF- α).

4. Isolation and screening of probiotics

Beneficial bacteria were isolated from the feces of healthy mice, and the 16S rDNA gene sequence was used to confirm that the obtained strains were safe and reliable and would not cause harm to the human body. By comparing the strains' ability to reduce the levels of inflammatory cytokines in the blood and liver, Lactobacillus johnsonii LBJ 456 was screened out for its ability to effectively reduce inflammation levels.

5. Introduction to Lactobacillus johnsonii LBJ 456

Academic achievements: more than 10 papers + 2 US patents + 1 EU patent + 3 Chinese patents (applied by Hehe Biotech)
The adhesion ability is as shown below (in vitro monolayer cell adhesion test):

LBJ 456 cells have good adhesion.
The results of the 60-day ultra-long colonization (human trial) are as follows:

LBJ 456 can still colonize the body after 60 days of consumption.
Innate resistance VS acquired embedding:

Microencapsulation has a good protective effect on bacteria.

Gastric acid tolerance:

The extremely strong gastric acid tolerance is an important basis for its successful adhesion and colonization. The gastric acid tolerance of Lactobacillus johnsonii LBJ456 and other common probiotics was compared under simulated gastric acid conditions of pH 3, 2, 1.6, and 1.2.
When the pH finally reached 1.2, although the number of Lactobacillus johnsonii LBJ 456 dropped by 3 orders of magnitude, it was the only surviving strain in the experiment.
Mucus binding proteins:

Mucus-binding protein is a cell surface protein containing a typical signal peptide, which is covalently linked to the bacterial cell wall. It is usually found in lactic acid bacteria. It can bind to intestinal mucin through glycosyl modification, thereby achieving adhesion and colonization.
Lactobacillus johnsonii LBJ456 has the highest number of MBPs, which explains to some extent the better adhesive colonization ability of this strain.

6. Verification of anti-inflammatory effect

The therapeutic effects of probiotics on rats with arthritis are as follows:

Lactobacillus johnsonii LBJ 456 can reduce the levels of inflammatory cytokines in the blood and liver while increasing the levels of some anti-inflammatory factors in the liver.

NK cells (natural killer cells) are an important factor in causing inflammation. Lactobacillus johnsonii LBJ 456 can also reduce the number of natural killer cells in the blood, spleen, and liver.

Lactobacillus johnsonii LBJ456 has a good inhibitory effect on Escherichia coli, Enterococcus faecalis, and Salmonella Enteritidis, and can inhibit the inflammatory response caused by pathogenic microorganisms to a certain extent.

references:

[1] Liu Cui, Du Xiaozheng, Jing Weiyao, et al. Explore the effects of electroacupuncture on intestinal flora and serum inflammatory factors in rabbits with a rheumatoid arthritis model based on the "gut-joint" axis [J/OL]. Chinese Traditional Medicine Information Journal, 1-8 [2024-01-10] https://doi.org/10.19879/j.cnki.1005-5304.202307047.
[2] Bungau SG, Behl T, Singh A, Sehgal A, Singh S, Chigurupati S, Vijayabalan S, Das S, Palanimuthu VR. Targeting Probiotics in Rheumatoid Arthritis. Nutrients. 2021 Sep 26;13(10):3376. doi : 10.3390/nu13103376. PMID: 34684377; PMCID: PMC8539185.
[3] Probiotic Gastrointestinal Transit and Colonization After Oral Administration: A Long Journey.
[4] Acid, Bile, and Heat Tolerance of Free and Microencapsulated Probiotic Bacteria.
[5] Comparative analysis of proteins with a mucus-binding domain found exclusively in lactic acid bacteria.

7. Analysis of excipients

Mulberries: Rich in anthocyanins and polyphenols, they are natural antioxidants. Anthocyanins can inhibit the expression of pro-inflammatory cytokines, cell adhesion molecules, and oxygen free radicals, affect the nuclear transcription factor KB and mitogen-activated protein kinase pathways, mediate the expression of nitric oxide and cyclooxygenase 2, and exert anti-inflammatory effects. inflammatory effect.
Concanavura: Canana peptide has a good inhibitory effect on COX-2. Cyclooxygenase-2 is an inducible cyclooxygenase, and its expression is significantly up-regulated when stimulated by inflammatory signals, thus aggravating the inflammatory response and causing cell damage. , causing cell senescence; plant-derived bioactive peptides can improve inflammation and cell senescence by down-regulating the expression of COX-2 mRNA.
Almonds: The anti-inflammatory effect of almonds is mainly due to the unsaturated fatty acids, polyphenols, and tocopherols contained in almond oil. Zorrilla et al. believe that almond oil rich in polyphenols can induce inflammatory cells in a rat colitis model. Infiltration is reduced and myeloperoxidase (MPO) activity and other related enzymes, such as iNOS, are inhibited. Research by Muller et al. shows that almond oil rich in oleic acid can significantly inhibit the activities of iNOS and COX-2, and reduce the level of the pro-inflammatory cytokine TNF-α to achieve anti-inflammatory purposes.
Inulin and fructooligosaccharides: commonly used prebiotics to promote the growth and metabolism of probiotics in the body.

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