Probioway's Lactobacillus rhamnosus powder is manufactured through a carefully controlled, multi-stage process to ensure maximum viability, purity, and stability. Our advanced production techniques adhere to GMP, ISO, and HACCP standards, guaranteeing a premium probiotic ingredient suitable for nutraceuticals, functional foods, and pharmaceuticals.
1. Strain Selection & Cultivation
Strain Activation: A master culture of L. rhamnosus is revived from a preserved stock and inoculated into a sterile growth medium.
Pre-Fermentation: The bacteria are pre-cultured in small batches to ensure optimal metabolic activity before large-scale fermentation.
Large-Scale Fermentation: The activated culture is transferred to industrial-scale fermenters containing a nutrient-rich medium (e.g., glucose, yeast extract, minerals). Fermentation conditions (pH, temperature, oxygen levels) are strictly controlled to maximize bacterial growth.
2. Harvesting & Concentration
Centrifugation: After fermentation, the bacterial biomass is separated from the growth medium using high-speed centrifugation, forming a dense cell paste.
Washing: The paste undergoes multiple washes with sterile buffer solutions to remove residual media components and metabolic byproducts.
3. Stabilization & Drying
Protective Formulation: The cell paste is mixed with cryoprotectants (e.g., trehalose, sucrose) to enhance survival during drying and storage.
Lyophilization (Freeze-Drying): The formulated slurry is frozen at ultra-low temperatures and subjected to vacuum drying, removing moisture while preserving bacterial viability. This step ensures long-term stability and shelf life.
Microencapsulation (Optional): For enhanced gastric acid resistance, some L. rhamnosus powder batches undergo microencapsulation with plant-based polymers (e.g., alginate, chitosan).
4. Milling & Blending
Particle Size Adjustment: The freeze-dried powder is milled to achieve a uniform particle size, improving solubility and ease of formulation.
Blending with Prebiotics (Optional): Synbiotic blends are created by combining L. rhamnosus powder with prebiotics (e.g., FOS, inulin) for enhanced probiotic activity.
5. Quality Control & Packaging
Viability Testing: CFU counts are verified via plate counting or flow cytometry to ensure potency meets specifications (e.g., 10–100 billion CFU/g).
Contaminant Screening: Tests for pathogens, heavy metals, and allergens confirm product safety.
Stability Testing: Accelerated shelf-life studies validate storage conditions (typically 2+ years at ≤25°C).
Aseptic Packaging: The powder is packed in moisture-resistant, nitrogen-flushed bags or airtight containers to prevent oxidation and humidity damage.
Probioway's Commitment to Excellence
Probioway's L. rhamnosus powder production combines scientific precision, cutting-edge technology, and rigorous quality control, delivering a probiotic ingredient with:
✔ High viability (>90% survival post-lyophilization)
✔ Excellent gastric acid and bile resistance
✔ Customizable CFU counts and formulations
Trust Probioway for premium L. rhamnosus powder tailored to your application needs. Contact us today to discuss your requirements!
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